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Mixing (reconstituting) a vial, step by step
The standard reconstitution technique, step by step, in terms of the supplies it actually takes.
Last reviewed · written by PepPrep · not medical advice
This is the same standard technique printed in pharmacy leaflets everywhere, described in terms of the supplies involved:
- Set up clean. Wash hands, put on gloves if you use them, and wipe the work surface with 70% alcohol.
- Swab both stoppers. One alcohol pad for the water vial's rubber top, a fresh one for the powder vial's. Let them air-dry a few seconds.
- Draw the water. Use a luer-lock syringe (a blunt fill needle makes this quick) and pull up your chosen amount — the calculator helps you pick a convenient one.
- Add it slowly, down the glass. Angle the needle so water runs down the inside wall of the powder vial instead of jetting onto the powder.
- Swirl, never shake. Gently roll or swirl until the liquid is clear. Shaking is the classic beginner mistake — delicate contents don't appreciate it.
- Label it. Write the date and the concentration (e.g. "mixed 8/29 — 2 mL") on tape or the label. Future-you will be grateful.
- Store it cold and dark, and put used needles in a sharps container — never household trash.
Supplies mentioned: water & vials · syringes · alcohol pads, gloves, sharps
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